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Article Dans Une Revue FEMS Microbiology Letters Année : 2021

Staphylococcus aureus membrane-damaging activities of four phenolics

Lynda Bouarab
  • Fonction : Auteur
Pascal Degraeve
Jalloul Bouajila
  • Fonction : Auteur
Amandine Cottaz
  • Fonction : Auteur
Fouzia Jbilou
  • Fonction : Auteur
Nadia Oulahal

Résumé

Abstract The membrane-damaging activities of four phenolics chosen for their bactericidal activity against Staphylococcus aureus CNRZ3 were investigated: 5,7-dihydroxy-4-phenylcoumarin (DHPC), 5,8-dihydroxy-1,4-naphthoquinone (DHNQ), epigallocatechin gallate (EGCG) and isobutyl 4-hydroxybenzoate (IBHB). Staphylococcus aureus CNRZ3 cells, as well as model liposomes mimicking its membrane phospholipids composition, were treated with each phenolic at its minimal bactericidal concentration. Membrane integrity, intracellular pH and intracellular esterase activity were examined by flow cytometric analysis of S. aureus cells stained with propidium iodide and SYTO® 9, 2′,7′-bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester, and 5(6)-carboxyfluorescein diacetate, respectively. While intracellular pH was affected by the foyr phenolics, only DHNQ and to a lesser extent EGCG, caused a loss of membrane integrity. Flow cytometric analysis of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and DPPC/POPG (2-oleoyl-1-palmitoyl-sn-glycero-3-phosphoglycerol) liposomes stained with Coumarin 6 (which penetrates the lipid bilayer) or 5-N(octadecanoyl)-amino-fluorescein (which binds to the liposome shell) suggested that only EGCG and DHNQ penetrated the bilayer of phospholipids of liposomes. Taken together, these findings support the hypothesis that EGCG and DHNQ bactericidal activity results from their accumulation in the phospholipid bilayer of S. aureus cells membrane causing its disruption.

Dates et versions

hal-04388602 , version 1 (11-01-2024)

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Citer

Lynda Bouarab, Pascal Degraeve, Jalloul Bouajila, Amandine Cottaz, Fouzia Jbilou, et al.. Staphylococcus aureus membrane-damaging activities of four phenolics. FEMS Microbiology Letters, 2021, 368 (13), ⟨10.1093/femsle/fnab081⟩. ⟨hal-04388602⟩
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