Use of Percoll for the infection of cells in vitro with Cryptosporidium parvum oocysts
Abstract
A method for the infection of non-adherent THP-1 cells and adherent MDBK cells with Cryptosporidium parÕum oocysts using isotonic Percoll solutions was developed. Excystation was maximal after 2 h, but toxicity increased with the oocystrcell ratio and the incubation time. The infection rates did not increase with the oocystrcell ratio and both cell types were equally parasitized. q
Domains
Parasitology
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