Use of Percoll for the infection of cells in vitro with Cryptosporidium parvum oocysts - Université Claude Bernard Lyon 1 Access content directly
Journal Articles Journal of Microbiological Methods Year : 2001

Use of Percoll for the infection of cells in vitro with Cryptosporidium parvum oocysts

Abstract

A method for the infection of non-adherent THP-1 cells and adherent MDBK cells with Cryptosporidium parÕum oocysts using isotonic Percoll solutions was developed. Excystation was maximal after 2 h, but toxicity increased with the oocystrcell ratio and the incubation time. The infection rates did not increase with the oocystrcell ratio and both cell types were equally parasitized. q

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Parasitology
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Dates and versions

hal-02110972 , version 1 (26-04-2019)

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Philippe Lawton, Roselyne Mancassola, Muriel Naciri, Anne-Françoise Pétavy. Use of Percoll for the infection of cells in vitro with Cryptosporidium parvum oocysts. Journal of Microbiological Methods, 2001, 46 (1), pp.81-84. ⟨10.1016/S0167-7012(01)00265-2⟩. ⟨hal-02110972⟩
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