%0 Journal Article %T Use of Percoll for the infection of cells in vitro with Cryptosporidium parvum oocysts %+ Institut des Sciences Pharmaceutiques et Biologiques (ISPB) %+ Station de Pathologie aviaire et parasitologie [Nouzilly] (PAP) %A Lawton, Philippe %A Mancassola, Roselyne %A Naciri, Muriel %A Pétavy, Anne-Françoise %< avec comité de lecture %@ 0167-7012 %J Journal of Microbiological Methods %I Elsevier %V 46 %N 1 %P 81-84 %8 2001 %D 2001 %R 10.1016/S0167-7012(01)00265-2 %K MDBK %K Percoll %K Cryptosporidium parvum %K Culture %K THP-1 %K In vitro %Z Life Sciences [q-bio]/Microbiology and Parasitology/ParasitologyJournal articles %X A method for the infection of non-adherent THP-1 cells and adherent MDBK cells with Cryptosporidium parÕum oocysts using isotonic Percoll solutions was developed. Excystation was maximal after 2 h, but toxicity increased with the oocystrcell ratio and the incubation time. The infection rates did not increase with the oocystrcell ratio and both cell types were equally parasitized. q %G English %2 https://univ-lyon1.hal.science/hal-02110972/document %2 https://univ-lyon1.hal.science/hal-02110972/file/14-Lawton%2001%20modifi%C3%A9.pdf %L hal-02110972 %U https://univ-lyon1.hal.science/hal-02110972 %~ UNIV-LYON1 %~ INRA %~ AGREENIUM %~ UDL %~ UNIV-LYON %~ INRAE %~ INFECTIOLOGIE-SANTE-PUBLIQUE