Inter-laboratory assessment of flow cytometric monocyte HLA-DR expression in clinical samples - Université Claude Bernard Lyon 1 Accéder directement au contenu
Article Dans Une Revue Cytometry Part B: Clinical Cytometry Année : 2013

Inter-laboratory assessment of flow cytometric monocyte HLA-DR expression in clinical samples

Résumé

Background: Diminished expression of human leukocyte antigen DR on circulating monocytes (mHLA-DR) is a reliable indicator of immunosuppression in critically ill patients, predictive of both adverse outcome and septic complications. The objective of the present work was to test, in an interlaboratory clinical study, a standardized protocol for mHLA-DR measurement by flow cytometry. Methods: mHLA-DR was assessed in fresh whole blood according to a standardized staining protocol. Cells were analyzed on different flow cytometers (FC500, Navios, FACS Canto II) in different laboratories (Lyon and Grenoble). Results were expressed as numbers of antibodies bound per cell (AB/C). Results: Correlations between results were excellent (Pearson and interclass correlation coefficients > 0.98). Coefficients of variations for intra-assay precision ranged from 1.9 to 3.2%. Conclusion: The present report highlights the robustness of this standardized flow cytometric protocol for mHLA-DR measurement in multicentric clinical studies.
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Dates et versions

hal-04060913 , version 1 (06-04-2023)

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Julie Demaret, Alexandre Walencik, Marie-Christine Jacob, Jean-François Timsit, Fabienne Venet, et al.. Inter-laboratory assessment of flow cytometric monocyte HLA-DR expression in clinical samples. Cytometry Part B: Clinical Cytometry, 2013, 84B (1), pp.59-62. ⟨10.1002/cyto.b.21043⟩. ⟨hal-04060913⟩
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