Mitochondrial threshold for H2O2 release in skeletal muscle of mammals - Université Claude Bernard Lyon 1 Accéder directement au contenu
Article Dans Une Revue Mitochondrion Année : 2020

Mitochondrial threshold for H2O2 release in skeletal muscle of mammals

Résumé

The aim of the study was to evaluate the interplay between mitochondrial respiration and H2O2 release during the transition from basal non-phosphorylating to maximal phosphorylating states. We conducted a large scale comparative study of mitochondrial oxygen consumption, H2O2 release and electron leak (% H2O2/O) in skeletal muscle mitochondria isolated from mammal species ranging from 7 g to 500 kg. Mitochondrial fluxes were measured at different steady state rates in presence of pyruvate, malate, and succinate as respiratory substrates. Every species exhibited a burst of H2O2 release from skeletal muscle mitochondria at a low rate of oxidative phosphorylation, essentially once the activity of mitochondrial oxidative phosphorylation reached 26% of the maximal respiration. This threshold for ROS generation thus appears as a general characteristic of skeletal muscle mitochondria in mammals. These findings may have implications in situations promoting succinate accumulation within mitochondria, such as ischemia or hypoxia.
Fichier principal
Vignette du fichier
S1567724920301677.pdf (789.55 Ko) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-02971414 , version 1 (22-08-2022)

Licence

Paternité - Pas d'utilisation commerciale

Identifiants

Citer

Yann Voituron, Mélanie Boël, Damien Roussel. Mitochondrial threshold for H2O2 release in skeletal muscle of mammals. Mitochondrion, 2020, 54, pp.85-91. ⟨10.1016/j.mito.2020.07.005⟩. ⟨hal-02971414⟩
54 Consultations
14 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More