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Journal Articles Mitochondrion Year : 2020

Mitochondrial threshold for H2O2 release in skeletal muscle of mammals

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Abstract

The aim of the study was to evaluate the interplay between mitochondrial respiration and H2O2 release during the transition from basal non-phosphorylating to maximal phosphorylating states. We conducted a large scale comparative study of mitochondrial oxygen consumption, H2O2 release and electron leak (% H2O2/O) in skeletal muscle mitochondria isolated from mammal species ranging from 7 g to 500 kg. Mitochondrial fluxes were measured at different steady state rates in presence of pyruvate, malate, and succinate as respiratory substrates. Every species exhibited a burst of H2O2 release from skeletal muscle mitochondria at a low rate of oxidative phosphorylation, essentially once the activity of mitochondrial oxidative phosphorylation reached 26% of the maximal respiration. This threshold for ROS generation thus appears as a general characteristic of skeletal muscle mitochondria in mammals. These findings may have implications in situations promoting succinate accumulation within mitochondria, such as ischemia or hypoxia.
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hal-02971414 , version 1 (22-08-2022)

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Attribution - NonCommercial - CC BY 4.0

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Yann Voituron, Mélanie Boël, Damien Roussel. Mitochondrial threshold for H2O2 release in skeletal muscle of mammals. Mitochondrion, 2020, 54, pp.85-91. ⟨10.1016/j.mito.2020.07.005⟩. ⟨hal-02971414⟩
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