Plant responses to heavy metals and their storage constitute a crucial step to understand the envi- ronmental impacts of metallic trace elements (MTEs). In controlled experiments, we previously demonstrated the tolerance and resilience of Japanese knotweed to soil artificial polymetallic contamination. Using the same experimental design, we tested here the effect of three individual MTEs on Fallopia × bohemica performance traits. Rhizome fragments from three different sites (considered as distinct morphotypes) were grown in a greenhouse for 1 month on a prairial soil artificially contaminated with either Cd, Cr (VI) or Zn at concen- trations corresponding to relatively highly polluted soils. Our results confirmed the high tolerance of Bohe- mian knotweed to metal stress, though, plant response to MTE pollution was dependant on MTE identity. Bohe- mian knotweed was stimulated by Cr (VI) (increased root and aerial masses), did not display any measurable change in performance traits under Cd at the high dose of 10 mg kg-1, and uptook all MTEs in its rhizome, but only Zn was transferred to its aerial parts. We also highlighted changes in root secondary metabolism that were more accentuated with Zn, including the increase of anthraquinone, stilbene and biphenyl derivatives. These results compared to multi-contamination experi- ments previously published suggest complex interac- tions between metals and plant, depending principally on metal identity and also suggest a potential role of soil microbes in the interactions.