Direct Identification of Staphylococcus aureus and Determination of Methicillin Susceptibility From Positive Blood-Culture Bottles in a Bact/ALERT System Using Binax Now \textitS. aureus and PBP2a Tests - Archive ouverte HAL Access content directly
Journal Articles Annals of Laboratory Medicine Year : 2015

Direct Identification of Staphylococcus aureus and Determination of Methicillin Susceptibility From Positive Blood-Culture Bottles in a Bact/ALERT System Using Binax Now \textitS. aureus and PBP2a Tests

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1
S. Heraud
  • Function : Author
A.-M. Freydiere
  • Function : Author
M. Bes
  • Function : Author
A. Tristan
  • Function : Author
F. Vandenesch
  • Function : Author
F. Laurent
  • Function : Author
O. Dauwalder
  • Function : Author

Abstract

Staphylococcus aureus bacteremia is associated with high mortality and morbidity, requiring prompt and appropriate antimicrobial treatment. Therefore, it is important to detect methicillin-resistant S. aureus (MRSA) rapidly from blood cultures. Two immunochromatographic tests, BinaxNow S. aureus and BinaxNow PBP2a, were directly applied to 79 Bact/Alert bottles that were positive for Gram positive cocci in cluster aggregations. Sensitivity and specificity for the identification of S. aureus and determination of methicillin resistance were 94% and 87%, and 100% and 100%, respectively, with less than 30 min of performance time. These tests are efficient and rapid; these tests are valuable alternatives to more sophisticated and expensive methods used in the diagnosis of MRSA bacteremia.

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hal-02487306 , version 1 (21-02-2020)

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S. Heraud, A.-M. Freydiere, Anne Doleans-Jordheim, M. Bes, A. Tristan, et al.. Direct Identification of Staphylococcus aureus and Determination of Methicillin Susceptibility From Positive Blood-Culture Bottles in a Bact/ALERT System Using Binax Now \textitS. aureus and PBP2a Tests. Annals of Laboratory Medicine, 2015, 35 (4), pp.454. ⟨10.3343/alm.2015.35.4.454⟩. ⟨hal-02487306⟩
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