Estimation of the RNU2 macrosatellite mutation rate by BRCA1 mutation tracing - Université Claude Bernard Lyon 1 Access content directly
Journal Articles Nucleic Acids Research Year : 2014

Estimation of the RNU2 macrosatellite mutation rate by BRCA1 mutation tracing

C. Tessereau
  • Function : Author
N. Monnet
  • Function : Author
M. Buisson
  • Function : Author
L. Barjhoux
  • Function : Author
M. Leone
  • Function : Author
B. Feng
  • Function : Author
D. E. Goldgar
  • Function : Author
O. M. Sinilnikova
  • Function : Author
S. Mousset
  • Function : Author
L. Duret
  • Function : Author
S. Mazoyer

Abstract

Large tandem repeat sequences have been poorly investigated as severe technical limitations and their frequent absence from the genome reference hinder their analysis. Extensive allelotyping of this class of variation has not been possible until now and their mutational dynamics are still poorly known. In order to estimate the mutation rate of a macrosatellite, we analysed in detail the RNU2 locus, which displays at least 50 different alleles containing 5-82 copies of a 6.1 kb repeat unit. Mining data from the 1000 Genomes Project allowed us to precisely estimate copy numbers of the RNU2 repeat unit using read depth of coverage. This further revealed significantly different mean values in various recent modern human populations, favoring a scenario of fast evolution of this locus. Its proximity to a disease gene with numerous founder mutations, BRCA1, within the same linkage disequilibrium block, offered the unique opportunity to trace RNU2 arrays over a large timescale. Analysis of the transmission of RNU2 arrays associated with one 'private' mutation in an extended kindred and four founder mutations in multiple kindreds gave an estimation by maximum likelihood of 5 x 10(-3) mutations per generation, which is close to that of microsatellites.

Dates and versions

hal-02045532 , version 1 (22-02-2019)

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Cite

C. Tessereau, Yann Lesecque, N. Monnet, M. Buisson, L. Barjhoux, et al.. Estimation of the RNU2 macrosatellite mutation rate by BRCA1 mutation tracing. Nucleic Acids Research, 2014, 42 (14), pp.9121-30. ⟨10.1093/nar/gku639⟩. ⟨hal-02045532⟩
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