Prostaglandin E2 enhancement of interferon-gamma production by antigen-stimulated type 1 helper T cells. - Université Claude Bernard Lyon 1 Accéder directement au contenu
Article Dans Une Revue Cellular Immunology Année : 1999

Prostaglandin E2 enhancement of interferon-gamma production by antigen-stimulated type 1 helper T cells.

Résumé

Prostaglandin E2 (PGE2) is a potent mediator generated in immune tissues by cyclooxygenation of arachidonic acid. PGE2 affects T cell functions through four homologous G protein-coupled receptors termed EP1R, EP2R, EP3R, and EP4R that differ in tissue distribution and signaling. Antigen-evoked secretion of interferon-gamma (IFN-gamma) by sperm whale myoglobin-specific Th1 cells of DBA/2 mouse I-Ed-restricted clones, that express EP3Rs and EP4Rs, was enhanced a maximum of 3-fold by 10(-10) to 10(-8) M PGE2 and 2.5-fold each for the EP1R/EP3R-directed agonist sulprostone (10(-8) and 10(-7) M) and for the EP4R/EP3R/EP2R agonist misoprostol (10(-9) M). Neither PGE2 nor the synthetic analogs affected secretion of IFN-gamma by PMA plus ionomycin-stimulated clones of Th1 cells. Antigen-evoked secretion of IFN-gamma by influenza hemagglutinin-specific mouse lymph node Th1 cells, that also express EP3Rs and EP4Rs, was increased a maximum of 12-fold by 10(-9) to 10(-8) M PGE2, 14-fold by 10(-9) M sulprostone, and 10-fold by 10(-9) M misoprostol. Production of IFN-gamma by either type of Th1 cell was not affected significantly by 10(-6) M PGE2 alone. The generation of IFN-gamma by antigen-stimulated Th1 cells thus is significantly enhanced by physiologically relevant concentrations of PGE2.

Domaines

Cancer

Dates et versions

hal-00641043 , version 1 (14-11-2011)

Identifiants

Citer

D. Bloom, N. Jabrane-Ferrat, L. Zeng, A. Wu, L. Li, et al.. Prostaglandin E2 enhancement of interferon-gamma production by antigen-stimulated type 1 helper T cells.. Cellular Immunology, 1999, 194 (1), pp.21-7. ⟨10.1006/cimm.1999.1479⟩. ⟨hal-00641043⟩
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