Phenotypic conversion of human mammary carcinoma cells by autocrine human growth hormone - Université Claude Bernard Lyon 1 Accéder directement au contenu
Article Dans Une Revue Proceedings of the National Academy of Sciences of the United States of America Année : 2004

Phenotypic conversion of human mammary carcinoma cells by autocrine human growth hormone

Résumé

We report here that autocrine production of human growth hormone (hGH) results in a phenotypic conversion of mammary carcinoma cells such that they exhibit the morphological and molecular characteristics of a mesenchymal cell, including expression of fibronectin and vimentin. Autocrine production of hGH resulted in reduced plakoglobin expression and relocalization of E-cadherin to the cytoplasm, leading to dissolution of cell-cell contacts and decreased cell height. These phenotypic changes were accompanied by an increase in cell motility, elevated activity of specific matrix metalloproteinases, and an acquired ability to invade a reconstituted basement membrane. Forced expression of plakoglobin significantly decreased mammary carcinoma cell migration and invasion stimulated by autocrine hGH. In vivo, autocrine hGH stimulated local invasion of mammary carcinoma cells concomitant with a prominent stromal reaction in comparison with well delineated and capsulated growth of mammary carcinoma cells lacking autocrine production of hGH. Thus, autocrine production of hGH by mammary carcinoma cells is sufficient for generation of an invasive phenotype. Therapeutic targeting of autocrine hGH may provide a mechanistic approach to prevent metastatic extension of human mammary carcinoma.

Domaines

Autre [q-bio.OT]

Dates et versions

hal-00069443 , version 1 (17-05-2006)

Identifiants

Citer

S. Mukhina, H.C. Mertani, K. Guo, K.O. Lee, P.D. Gluckman, et al.. Phenotypic conversion of human mammary carcinoma cells by autocrine human growth hormone. Proceedings of the National Academy of Sciences of the United States of America, 2004, 101, pp.15166-15171. ⟨10.1073/pnas.0405881101⟩. ⟨hal-00069443⟩
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