%0 Journal Article %T Analysis of Frankia evolutionary radiation using glnII sequences %+ Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM) %A Cournoyer, B. %A Lavire, Céline %< avec comité de lecture %@ 0378-1097 %J FEMS Microbiology Letters %I Wiley-Blackwell %V 177 %N 1 %P 29-34 %8 2006 %D 2006 %R 10.1111/j.1574-6968.1999.tb13709.x %M 10436920 %K evolution %K actinomycete %K symbiosis %K nodule %K glutamine synthetase %Z Life Sciences [q-bio]Journal articles %X Using a glnII (encoding glutamine synthetase II) PCR selective screening, a Frankia ACN14a gene library clone was isolated. A derived glnII-hybridising 2.7-kb HindIII subclone was characterised. Identities of 95% and 93% were observed, respectively, with the corresponding Frankia CpI1 glnI and glnII regions. A variable segment of the glnII region was selected, PCR amplified from various Frankia genomes, sequenced, and used to investigate phylogenetic relationships within the genus. glnII phylogenetic inferences are well-resolved and allowed us to deduce evolutionary trends among Frankia. Frankia radiation seems to begin with a diversification according to the ability or not to infect actinorhizal plants. The infective strains are divided into two clusters matching plant-colonising specificities. %G English %L hal-02569778 %U https://univ-lyon1.hal.science/hal-02569778 %~ CNRS %~ UNIV-LYON1 %~ INRA %~ BIOENVIS %~ AGREENIUM %~ UDL %~ UNIV-LYON %~ INRAE %~ ECOMIC