Comparison of prominent Azospirillum strains in Azospirillum–Pseudomonas–Glomus consortia for promotion of maize growth - Université Claude Bernard Lyon 1 Accéder directement au contenu
Article Dans Une Revue Applied Microbiology and Biotechnology Année : 2013

Comparison of prominent Azospirillum strains in Azospirillum–Pseudomonas–Glomus consortia for promotion of maize growth

Résumé

Azospirillum are prominent plant growth-promoting rhizobacteria (PGPR) extensively used as phytostimulatory crop inoculants, but only few studies are dealing with Azospirillum -containing mixed inocula involving more than two microorganisms. We compared here three prominent Azospirillum strains as part of three-component consortia including also the PGPR Pseudomonas fluorescens F113 and a mycorrhizal inoculant mix composed of three Glomus strains. Inoculant colonization of maize was assessed by quantitative PCR, transcription of auxin synthesis gene ipdC (involved in phytostimulation) in Azospirillum by RT-PCR, and effects on maize by secondary metabolic profiling and shoot biomass measurements. Results showed that phytostimulation by all the three-component consortia was comparable, despite contrasted survival of the Azospirillum strains and different secondary metabolic responses of maize to inoculation. Unexpectedly, the presence of Azospirillum in the inoculum resulted in lower phytostimulation in comparison with the Pseudomonas Glomus two-component consortium, but this effect was transient. Azospirillum 's ipdC gene was transcribed in all treatments, especially with three-component consortia, but not with all plants and samplings. Inoculation had no negative impact on the prevalence of mycorrhizal taxa in roots. In conclusion, this study brought new insights in the functioning of microbial consortia and showed that Azospirillum Pseudomonas Glomus three-component inoculants may be useful in environmental biotechnology for maize growth promotion.

Domaines

Biotechnologies

Dates et versions

hal-02522189 , version 1 (27-03-2020)

Identifiants

Citer

Olivier Couillerot, A. Ramirez-Trujillo, Vincent Walker, A. Von Felten, J. Jansa, et al.. Comparison of prominent Azospirillum strains in Azospirillum–Pseudomonas–Glomus consortia for promotion of maize growth. Applied Microbiology and Biotechnology, 2013, 97 (10), pp.4639-4649. ⟨10.1007/s00253-012-4249-z⟩. ⟨hal-02522189⟩
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