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Article Dans Une Revue Journal of Microbiology Research Année : 2017

Nitrogenase and Hydrogenase of the Actinobacteria Frankia: From Gene Expression to Proteins Function

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Frankia R43 is a filamentous actinobacteria, known for its ability to fix atmospheric nitrogen and to concomitantly produce hydrogen. Not much is understood about the variations in nitrogen fixation and hydrogen production among Frankia strains at the molecular level. Thus, studies providing direct comparisons between less known and well-annotated Frankia strains, may provide further information on the specific functions of the nitrogenase and hydrogenase(s). We compared candidate gene expression, protein sequence similarities and physiological activities of the well-annotated clade I Frankia casuarinae strain CcI3 and the recently sequenced lineage III Frankia strain R43. Our candidate genes, members of the nif gene cluster, involved in dinitrogen fixation, and genes encoding uptake hydrogenase (Hup) subunits, with an importance for energetic efficiency, were examined under varying oxygen and nitrogen conditions. Relative expression of almost all genes tested was higher in CcI3 than in R43, except nifK, following different but distinct expression patterns among all conditions. Candidate genes expression in CcI3 seemed more sensitive to oxygen than in R43, and the oxygen status seemed to influence gene expression much more than the nitrogen status per se.
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hal-02487200 , version 1 (21-02-2020)

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Kerstin H. Richau, Petar Pujic, Philippe Normand, Anita Sellstedt. Nitrogenase and Hydrogenase of the Actinobacteria Frankia: From Gene Expression to Proteins Function. Journal of Microbiology Research, 2017, 7 (4), pp.79-92. ⟨10.5923/j.microbiology.20170704.01⟩. ⟨hal-02487200⟩
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