In vitro and in vivo anti-melanoma effects of Daphne gnidium aqueous extract via activation of the immune system - Université Claude Bernard Lyon 1 Access content directly
Journal Articles Tumor Biology Year : 2016

In vitro and in vivo anti-melanoma effects of Daphne gnidium aqueous extract via activation of the immune system

Mounira Krifa
  • Function : Author
Kamel Ghedira
  • Function : Author
Leila Chekir-Ghedira
  • Function : Author
Fadwa Chaabane
  • Function : Author
Nadia Mustapha
  • Function : Author
Imen Mokdad-Bzeouich
  • Function : Author
Aicha Sassi
  • Function : Author
Soumaya Kilani-Jaziri
  • Function : Author
Mayssa Fathallah
  • Function : Author

Abstract

The purpose of this study was to assess the antitumor and immunomodulatory effects of the aqueous extract from Daphne gnidium in mice-bearing melanoma tumor. Balb/C mice were subcutaneously implanted with B16-F10 cells and treated intraperitoneally with the aqueous extract at 200 mg/Kg b.w for 21 days. After euthanization on day 22, the tumors were weighed; lymphocyte proliferation, cytotoxic T lymphocyte (CTL), and natural killer (NK) cell activities were evaluated using the MTT assay. Macrophage phagocytosis was studied by measuring the lysosomal activity. In addition to its potential to inhibit the growth of the transplantable tumor, the aqueous extract remarkably induced splenocyte proliferation and both NK and CTL activities in tumor-bearing mice. The aqueous extract was also seen to have promoted lysosomal activity of host macrophages.
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Dates and versions

hal-02466013 , version 1 (04-02-2020)

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Mounira Krifa, Kamel Ghedira, Leila Chekir-Ghedira, Fadwa Chaabane, Nadia Mustapha, et al.. In vitro and in vivo anti-melanoma effects of Daphne gnidium aqueous extract via activation of the immune system. Tumor Biology, 2016, 37 (5), pp.6511-6517. ⟨10.1007/s13277-015-4492-x⟩. ⟨hal-02466013⟩
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