Water Dynamics in Shewanella oneidensis at Ambient and High Pressure using Quasi-Elastic Neutron Scattering - Archive ouverte HAL Access content directly
Journal Articles Scientific Reports Year : 2016

Water Dynamics in Shewanella oneidensis at Ambient and High Pressure using Quasi-Elastic Neutron Scattering

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Fabrizia Foglia
  • Function : Author
Rachael Hazael
  • Function : Author
Giovanna G. Simeoni
  • Function : Author
Marie-Sousai Appavou
  • Function : Author
Martine Moulin
  • Function : Author
Michael Haertlein
V. Trevor Forsyth
  • Function : Author
Tilo Seydel
Filip Meersman
  • Function : Author
Paul F. Mcmillan
  • Function : Author

Abstract

Quasielastic neutron scattering (QENS) is an ideal technique for studying water transport and relaxation dynamics at pico-to nanosecond timescales and at length scales relevant to cellular dimensions. Studies of high pressure dynamic effects in live organisms are needed to understand Earth's deep biosphere and biotechnology applications. Here we applied QENS to study water transport in Shewanella oneidensis at ambient (0.1 MPa) and high (200 MPa) pressure using H/D isotopic contrast experiments for normal and perdeuterated bacteria and buffer solutions to distinguish intracellular and transmembrane processes. The results indicate that intracellular water dynamics are comparable with bulk diffusion rates in aqueous fluids at ambient conditions but a significant reduction occurs in high pressure mobility. We interpret this as due to enhanced interactions with macromolecules in the nanoconfined environment. Overall diffusion rates across the cell envelope also occur at similar rates but unexpected narrowing of the QENS signal appears between momentum transfer values Q = 0.7-1.1 angstrom(-1) corresponding to real space dimensions of 6-9 angstrom. The relaxation time increase can be explained by correlated dynamics of molecules passing through Aquaporin water transport complexes located within the inner or outer membrane structures.
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hal-02331143 , version 1 (31-12-2020)

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Attribution - NoDerivatives - CC BY 4.0

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Fabrizia Foglia, Rachael Hazael, Giovanna G. Simeoni, Marie-Sousai Appavou, Martine Moulin, et al.. Water Dynamics in Shewanella oneidensis at Ambient and High Pressure using Quasi-Elastic Neutron Scattering. Scientific Reports, 2016, 6, ⟨10.1038/srep18862⟩. ⟨hal-02331143⟩
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