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Journal Articles Immunobiology Year : 2016

Characterization of a novel canine T-cell line established from a spontaneously occurring aggressive T-cell lymphoma with large granular cell morphology

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C. Bonnefont-Rebeix
  • Function : Author
Frédérique Ponce
  • Function : Author
  • PersonId : 1024196
D. Watrelot
  • Function : Author
S. E. Bouteille
  • Function : Author
S. Rapiteau
  • Function : Author
D. Razanajaona-Doll
  • Function : Author
J. J. Pin
  • Function : Author
Thierry Marchal
  • Function : Author
  • PersonId : 1039786

Abstract

Dogs with lymphoma are established as good model for human non-Hodgkin lymphoma studies. Canine cell lines derived from lymphomas may be valuable tools for testing new therapeutic drugs. In this context, we established a canine T-cell line, PER-VAS, from a primary aggressive T-cell lymphoma with large granular morphology. Flow cytometric analysis revealed a stable immunophenotype: PER-VAS cells were positively labelled for CD5, CD45, MHC II and TLR3, and were negative for CD3, CD4 and CD8 expression. Although unstable along the culture process, IL-17 and MMP12 proteins were detectable as late as at passages 280 and 325i.e. respectively 24 and 29 months post isolation. At passage 325, PER-VAS cells maintained the expression of IL-17, CD3, CD56, IFNgamma and TNFalpha mRNAs as shown by RT-PCR analysis. Stable rearrangement of the TCRgamma gene has been evidenced by PCR. PER-VAS cells have a high proliferation index with a doubling time of 16.5h and were tumorigenic in Nude mice. Compared to the canine cell lines already reported, PER-VAS cells display an original expression pattern, close to NKT cells, which makes them valuable tools for in vitro comparative research on lymphomas.
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hal-02192918 , version 1 (27-05-2020)

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Attribution - NonCommercial - NoDerivatives - CC BY 4.0

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C. Bonnefont-Rebeix, C. Fournel-Fleury, Frédérique Ponce, S. Belluco, D. Watrelot, et al.. Characterization of a novel canine T-cell line established from a spontaneously occurring aggressive T-cell lymphoma with large granular cell morphology. Immunobiology, 2016, 221 (1), pp.12-22. ⟨10.1016/j.imbio.2015.08.007⟩. ⟨hal-02192918⟩
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