%0 Journal Article %T Toxoplasma gondii: Identification and immune response against a group of proteins involved in cellular invasion %+ Université Claude Bernard Lyon 1 (UCBL) %+ Universidad de Granada = University of Granada (UGR) %+ Institut des Sciences Pharmaceutiques et Biologiques (ISPB) %A Azzouz, Samira %A Maache, Mimoun %A Osuna, Antonio %A Lawton, Philippe %A Pétavy, Anne Françoise %< avec comité de lecture %@ 0014-4894 %J Experimental Parasitology %I Elsevier %V 130 %N 1 %P 63-68 %8 2012 %D 2012 %R 10.1016/j.exppara.2011.09.012 %K Cellular invasion %K Toxoplasma gondii %K Permeability %K Vaccine %Z Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology %Z Life Sciences [q-bio]/Immunology/VaccinologyJournal articles %X Toxoplasma gondii is an ubiquitous intracellular parasite, causative agent of toxoplasmosis, and a worldwide zoonosis for which an effective vaccine is needed. A group of proteins secreted by tachyzoites during host-cell invasion was isolated from the interaction medium. It induced the permeability of the cells as assessed by alpha-sarcin and consequently facilitated the entry of the parasite into the cells. SDS-PAGE of the purified proteins showed a pattern of four proteins of 67, 42, 32 and 27 kDa. MRC-5 cells incubated with the total protein and the different electroeluted bands endured a high cellular death in presence of alpha-sarcin. BALb/C mice immunized with the group of proteins had a mixed Th1/Th2 response and were protected upon challenge with the parasites. %G English %2 https://univ-lyon1.hal.science/hal-02111025/document %2 https://univ-lyon1.hal.science/hal-02111025/file/19-Azzouz%2012%20modifi%C3%A9.pdf %L hal-02111025 %U https://univ-lyon1.hal.science/hal-02111025 %~ UNIV-LYON1 %~ UDL %~ UNIV-LYON