%0 Journal Article %T In vitro cultivation of Cryptosporidium parvum in the non-adherent human monocytic THP-1 cell line %+ Laboratoire de Parasitologie %+ Station de Pathologie aviaire et parasitologie [Nouzilly] (PAP) %A Lawton, Philippe %A Naciri, Muriel %A Mancassola, Roselyne %A Pétavy, Anne-Francoise %< avec comité de lecture %@ 1066-5234 %J Journal of Eukaryotic Microbiology %I Wiley %V 44 %N s6 %P 66S %8 1997 %D 1997 %R 10.1111/j.1550-7408.1997.tb05783.x %Z Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology %Z Life Sciences [q-bio]/BiotechnologyJournal articles %X This study shows that the human monocytic cell line THP-1 supports the growth of C. parvum. Immunofluorescence controls showed that only scarce oocysts remained after infection and disappeared Within the first 24 h of culture. A continuous asexual life cycle proceeded throughout the experiments, with at least 15d cultures. This model provides a useful tool for studies on the biology of C. parvum in cells involved in its transport in immunocompromised host %G English %2 https://univ-lyon1.hal.science/hal-02110909/document %2 https://univ-lyon1.hal.science/hal-02110909/file/9-Lawton%2097%20modifi%C3%A9.pdf %L hal-02110909 %U https://univ-lyon1.hal.science/hal-02110909 %~ UNIV-LYON1 %~ INRA %~ AGREENIUM %~ UDL %~ UNIV-LYON %~ INRAE %~ INFECTIOLOGIE-SANTE-PUBLIQUE