Reinforcement of STAT3 activity reprogrammes human embryonic stem cells to naive-like pluripotency - Université Claude Bernard Lyon 1 Accéder directement au contenu
Article Dans Une Revue Nature Communications Année : 2015

Reinforcement of STAT3 activity reprogrammes human embryonic stem cells to naive-like pluripotency

Hongwei Chen
  • Fonction : Auteur
Irène Aksoy
Jiaxuan Chen
  • Fonction : Auteur
Céline Vallot
Michael Weber
Lawrence W Stanton
  • Fonction : Auteur
Bertrand Pain

Résumé

Leukemia inhibitory factor (LIF)/STAT3 signalling is a hallmark of naive pluripotency in rodent pluripotent stem cells (PSCs), whereas fibroblast growth factor (FGF)-2 and activin/nodal signalling is required to sustain self-renewal of human PSCs in a condition referred to as the primed state. It is unknown why LIF/STAT3 signalling alone fails to sustain pluripotency in human PSCs. Here we show that the forced expression of the hormone-dependent STAT3-ER (ER, ligand-binding domain of the human oestrogen receptor) in combination with 2i/LIF and tamoxifen allows human PSCs to escape from the primed state and enter a state characterized by the activation of STAT3 target genes and long-term self-renewal in FGF2-and feeder-free conditions. These cells acquire growth properties, a gene expression profile and an epigenetic landscape closer to those described in mouse naive PSCs. Together, these results show that temporarily increasing STAT3 activity is sufficient to reprogramme human PSCs to naive-like pluripotent cells.
Fichier principal
Vignette du fichier
ncomms8095.pdf (3.1 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

hal-01997120 , version 1 (24-02-2020)

Identifiants

Citer

Hongwei Chen, Irène Aksoy, Fabrice Gonnot, Pierre Osteil, Maxime Aubry, et al.. Reinforcement of STAT3 activity reprogrammes human embryonic stem cells to naive-like pluripotency. Nature Communications, 2015, 6 (1), ⟨10.1038/ncomms8095⟩. ⟨hal-01997120⟩
99 Consultations
134 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More