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Article Dans Une Revue Journal of Cystic Fibrosis: Official Journal of the European Cystic Fibrosis Society Année : 2014

Neutrophil elastase-mediated increase in airway temperature during inflammation

Résumé

BACKGROUND: How elevated temperature is generated during airway infections represents a hitherto unresolved physiological question. We hypothesized that innate immune defence mechanisms would increase luminal airway temperature during pulmonary infection. METHODS: We determined the temperature in the exhaled air of cystic fibrosis (CF) patients. To further test our hypothesis, a pouch inflammatory model using neutrophil elastase-deficient mice was employed. Next, the impact of temperature changes on the dominant CF pathogen Pseudomonas aeruginosa growth was tested by plating method and RNAseq. RESULTS: Here we show a temperature of ~38°C in neutrophil-dominated mucus plugs of chronically infected CF patients and implicate neutrophil elastase:α1-proteinase inhibitor complex formation as a relevant mechanism for the local temperature rise. Gene expression of the main pathogen in CF, P. aeruginosa, under anaerobic conditions at 38°C vs 30°C revealed increased virulence traits and characteristic cell wall changes. CONCLUSION: Neutrophil elastase mediates increase in airway temperature, which may contribute to P. aeruginosa selection during the course of chronic infection in CF.

Dates et versions

hal-01953726 , version 1 (13-12-2018)

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Annika Schmidt, Azzaq Belaaouaj, Rosi Bissinger, Garrit Koller, Laurette Malleret, et al.. Neutrophil elastase-mediated increase in airway temperature during inflammation. Journal of Cystic Fibrosis: Official Journal of the European Cystic Fibrosis Society, 2014, 13 (6), pp.623-631. ⟨10.1016/j.jcf.2014.03.004⟩. ⟨hal-01953726⟩
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