Development of an elastography bench for histology and engineered tissue study-Preliminary results - Université Claude Bernard Lyon 1 Accéder directement au contenu
Communication Dans Un Congrès Année : 2017

Development of an elastography bench for histology and engineered tissue study-Preliminary results

Hamza Raki
  • Fonction : Auteur
  • PersonId : 19812
  • IdHAL : hamza-raki
Olivier Beuf
Simon A Lambert

Résumé

To study viscoelastic parameters of small ex vivo or engineered tissue samples, Magnetic Resonance Elastography (MRE) has been used during the last few years [1], [2]. It should enable a cautious sample-handling and be performed with a high spatial resolution. We describe here an elastography bench responding to those criteria. The setup is designed to image small samples (Fig.1). This requires an MRI coil with a high uniformity and filling factor to maximize the signal to noise ratio (SNR). Moreover the sample must be placed in the center of the coil and in contact with the mechanical transducer. Here, the sample holder is designed to slide into a 3D-printed support in which is included a Helmholtz coil tuned with two capacitor trimmers. Matching of the Helmholtz coil is done inductively using a circular coaxial coupling loop tuned at 200MHz. The sample holder is stopped at the center of the coil, guarantying the best RF uniformity and SNR. A cactus needle pierces the sample and is actuated by an MRI-compatible piezoelectric driver at 600Hz. Two samples were used to test the bench. The first one is made of polymerized fibrinogen (Sigma-F8630), which is used for engineered tissue. To limit motion of the sample, it was surrounded by a stiffer gel (DTM 133460). We also used a healthy rat brain embedded in agarose (Sigma A9414). The two embedding gels have well characterized mechanical properties and can serve as a reference.Acquisition of a FLASH and a MRE-compatible RARE sequence were made with a Bruker 4.7T scanner. Reconstruction of the viscoelastic parameters was done using an adapted method from Sinkus et al. [3].A voxel of 0.312x0.312x0.625mm3 with an SNR of 82.8 and 63.6 were obtained with the FLASH sequence, for the rat brain (Fig.2) and the phantom (Fig.3), respectively. Mean displacement amplitude for the RARE sequence was evaluated at 1.5µm and 5.6µm. Storage modulus representing elasticity was 1.8kPa and 1.4kPa. The gel surrounding the sample had an elasticity of 2.6kPa. Setup was easy to handle. It can be easily adapted to any MRI system. SNR is high and the induced mechanical displacement is enough to reconstruct viscoelastic parameters and highlight differences in elasticity between the commercial gel and the fibrinogen sample. Ex vivo results in brain are in agreement with literature [4].AcknowledgementThis work was supported by the LABEX PRIMES (ANR-XX-LABX-0063) of Université de Lyon, within the program "Investissements d'Avenir" (ANR-11-IDEX-0007) operated by the French National Research Agency (ANR).PEPS CNRS “Balanced”.References1-Boulet, J. Neurosci. Methods, 20112-Guertler, Proc. ISMRM, 20173-Sinkus, CR Mécanique, 20104-Millward, J. Neuroimmunol, 2014
20170707_abstractCNIV_final.pdf (385.48 Ko) Télécharger le fichier
21.CNIV_Bigot.pdf (2.43 Mo) Télécharger le fichier
image.png (16.66 Ko) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-01881205 , version 1 (02-10-2018)

Identifiants

  • HAL Id : hal-01881205 , version 1

Citer

Mathilde Bigot, Hugo Dorez, Celine Mandon, Christophe A. Marquette, Kevin Tse Ve Koon, et al.. Development of an elastography bench for histology and engineered tissue study-Preliminary results. Congrès National d'Imagerie du Vivant, Nov 2017, Paris, France. ⟨hal-01881205⟩
180 Consultations
23 Téléchargements

Partager

Gmail Facebook X LinkedIn More