Functional metagenomics: construction and high-throughput screening of fosmid libraries for discovery of novel carbohydrate-active enzymes - Université Claude Bernard Lyon 1 Accéder directement au contenu
Chapitre D'ouvrage Année : 2016

Functional metagenomics: construction and high-throughput screening of fosmid libraries for discovery of novel carbohydrate-active enzymes

Résumé

Activity-based metagenomics is one of the most efficient approaches to boost the discovery of novel biocatalysts from the huge reservoir of uncultivated bacteria. In this chapter, we describe a highly generic procedure of metagenomic library construction and high-throughput screening for carbohydrate-active enzymes. Applicable to any bacterial ecosystem, it enables the swift identification of functional enzymes that are highly efficient, alone or acting in synergy, to break down polysaccharides and oligosaccharides.
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Dates et versions

hal-01266523 , version 1 (02-02-2016)

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Lisa Ufarté, Sophie Bozonnet, Elisabeth Laville, Davide Cecchini, Sandra Serin, et al.. Functional metagenomics: construction and high-throughput screening of fosmid libraries for discovery of novel carbohydrate-active enzymes. Microbial Environmental Genomics MEG, 1399 (1ère ed.), Springer, 321 p., 2016, Methods in Molecular Biology, 978-1-4939-3367-9. ⟨10.1007/978-1-4939-3369-3_15⟩. ⟨hal-01266523⟩
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