Biocompatible photoresistant far-red emitting, fluorescent polymer probes, with near-infrared two-photon absorption, for living cell and zebrafish embryo imaging - Université Claude Bernard Lyon 1 Accéder directement au contenu
Article Dans Une Revue Biomaterials Année : 2015

Biocompatible photoresistant far-red emitting, fluorescent polymer probes, with near-infrared two-photon absorption, for living cell and zebrafish embryo imaging

Audrey Thomas
  • Fonction : Auteur
Julien Massin
Nadine Peyriéras
  • Fonction : Auteur
Chantal Andraud
  • Fonction : Auteur
  • PersonId : 829281

Résumé

Exogenous probes with far-red or near-infrared (NIR) two-photon absorption and fluorescence emission are highly desirable for deep tissue imaging while limiting autofluorescence. However, molecular probes exhibiting such properties are often hydrophobic. As an attractive alternative, we synthesized water-soluble polymer probes carrying multiple far-red fluorophores and demonstrated here their potential for live cell and zebrafish embryo imaging. First, at concentrations up to 10 μm, these polymer probes were not cytotoxic. They could efficiently label living HeLa cells, T lymphocytes and neurons at an optimal concentration of 0.5 μm. Moreover, they exhibited a high resistance to photobleaching in usual microscopy conditions. In addition, these polymer probes could be successfully used for in toto labeling and in vivo two-photon microscopy imaging of developing zebrafish embryos, with remarkable properties in terms of biocompatibility, internalization, diffusion, stability and wavelength emission range. The near-infrared two-photon absorption peak at 910 nm is particularly interesting since it does not excite the zebrafish endogenous fluorescence and is likely to enable long-term time-lapse imaging with limited photodamage.
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Dates et versions

hal-01142603 , version 1 (28-04-2022)

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Paternité - Pas d'utilisation commerciale

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Salim Adjili, Arnaud Favier, Guillaume Fargier, Audrey Thomas, Julien Massin, et al.. Biocompatible photoresistant far-red emitting, fluorescent polymer probes, with near-infrared two-photon absorption, for living cell and zebrafish embryo imaging. Biomaterials, 2015, 46, pp.70-81. ⟨10.1016/j.biomaterials.2014.12.026⟩. ⟨hal-01142603⟩
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