Microconductometric immunosensor for label-free and sensitive detection of Gram-negative bacteria. - Université Claude Bernard Lyon 1 Accéder directement au contenu
Article Dans Une Revue Biosensors and Bioelectronics Année : 2014

Microconductometric immunosensor for label-free and sensitive detection of Gram-negative bacteria.

Résumé

: Blood safety is a global health goal. In developed countries, bacterial contamination of platelet concentrates is the highest infectious risk in transfusion despite the current preventive strategies. We aimed to develop a conductometric biosensor for the generic, rapid and sensitive detection of Gram-negative bacteria. Our strategy is based on immunosensors: addressable magnetic nanoparticles coupled with anti-LPS antibodies were used for the generic capture of Gram-negative bacteria. Bacterial capture was characterized by impedancemetric and microscopic measurements. The results obtained with conductometric measurements allowed real-time, sensitive detection of Escherichia coli or Serratia marcescens cultures from 1 to 10(3)CFUmL(-1). The ability of the immunosensor to detect Gram negative bacteria was also tested on clinically relevant strains. The conductometric immunosensor allowed the direct detection of 10-10(3)CFUmL(-1) of Pseudomonas aeruginosa and Acinetobacter baumannii strains that were undetectable using standard immunoblot methods. Results showed that the conductometric response was not inhibited in 1% serum.
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Dates et versions

hal-00915124 , version 1 (06-12-2013)

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Sarra El Ichi, Fanny Leon, Ludivine Vossier, Hélène Marchandin, Abdelhamid Errachid, et al.. Microconductometric immunosensor for label-free and sensitive detection of Gram-negative bacteria.. Biosensors and Bioelectronics, 2014, 54, pp.378-384. ⟨10.1016/j.bios.2013.11.016⟩. ⟨hal-00915124⟩
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