Telomere deregulations possess cytogenetic, phenotype, and prognostic specificities in acute leukemias. - Université Claude Bernard Lyon 1 Accéder directement au contenu
Article Dans Une Revue Experimental Hematology Année : 2011

Telomere deregulations possess cytogenetic, phenotype, and prognostic specificities in acute leukemias.

Résumé

OBJECTIVE: Telomeres are protected by tightly regulated factors and elongated by telomerase. Short and/or deprotected chromosomes are recombinogenic and thereby cancer prone. MATERIALS AND METHODS: Together with the quantification of telomerase activity (TA), measuring telomere length (TL) and expression of the genes that govern telomere protection and elongation are useful for assessing telomere homeostasis. RESULTS: By these means we demonstrate that TL, hTERT, and TA are in the order acute myelogenous leukemia (AML) > T-cell acute lymphoblastic leukemia (T-ALL) > B-cell acute lymphoblastic leukemia (B-ALL) > T-ALL > AML, and B-ALL > AML > T-ALL. AML0 and AML3 display the lowest amounts of hTERT transcripts, and ALL and AML cells with cytogenetic abnormalities possess the shortest telomeres. hTERT expression includes phenotype-specific RNA maturation and correlates with TA but not with TL. A wide ratio of TA to hTERT expression between leukemia subtypes suggests phenotype-specific hTERT post-transcriptional deregulations. B- and T-ALL overexpress Ku70 and Pinx1, T-ALL PTOP and RAP1, and B-ALL TRF2, the expression of which is significantly higher in cases with abnormal karyotype. hTERT transcription and TL correlate with response to intensive chemotherapy, and hTERT and RAD50 are independent prognostic factors for survival. CONCLUSIONS: Each leukemia subtype possesses specific telomere dysregulations that rely on phenotype, karyotype, response to treatment, and survival.

Domaines

Cancer

Dates et versions

hal-00787970 , version 1 (13-02-2013)

Identifiants

Citer

Valérie Capraro, Linda Zane, Delphine Poncet, David Pérol, Perrine Galia, et al.. Telomere deregulations possess cytogenetic, phenotype, and prognostic specificities in acute leukemias.. Experimental Hematology, 2011, 39 (2), pp.195-202.e2. ⟨10.1016/j.exphem.2010.10.008⟩. ⟨hal-00787970⟩
95 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More