Adult T-cell leukaemia/lymphoma-like human T-cell leukaemia virus-1 replication in infective dermatitis.
Résumé
Adult T-cell leukaemia/lymphoma (ATLL) is a malignant T-cell proliferation that occurs in 3-5% of individuals infected with human T-cell leukaemia virus-1 (HTLV-1). HTLV-1 infection is also linked to the development of infective dermatitis (ID), an exudative dermatitis of children that has been proposed as a cofactor of ATLL. Here, HTLV-1 replication was investigated over time in a girl with ID and multiparasitic infestation including strongyloidiasis, a disease also known to predispose HTLV-1 carriers to ATLL. Quantitative polymerase chain reaction (PCR) revealed extremely high proviral loads. During the 2-year period of the present study, the proportion of circulating infected cells ranged between 12% and 36%. Quadruplicate linker-mediated PCR amplification of HTLV-1 flanking sequences identified a pattern of extensive and persistent oligoclonal expansion of infected lymphocytes. As viral loads, both the number and the degree of infected T-cell expansion were independent of treatment or clinical signs. However, the temporal fluctuation of proviral loads correlated significantly with the degree of infected T-cell expansion, but not with the overall number of detected clones. This pattern of HTLV-1 replication over time is very different from that observed in asymptomatic carriers and reminiscent of that observed in ATLL, a result consistent with the proposal of ID as an ATLL cofactor.
Mots clés
Antibodies
Viral/blood
Antiviral Agents/therapeutic use
Blotting
Western/methods
Cell Division
Child
Clone Cells
Dermatitis/*immunology/parasitology/*virology
Female
Human T-lymphotropic virus 1/immunology/*physiology
Humans
Lamivudine/therapeutic use
Leukemia-Lymphoma
T-Cell
Acute
HTLV-I-Associated/*immunology/parasitology
Parasitic Diseases/immunology/virology
Polymerase Chain Reaction/methods
Regression Analysis
Research Support
Non-U.S. Gov't
T-Lymphocytes/*virology
Viral Load
Virus Replication