Human serum albumin in the presence of AGuIX nanoagents: Structure stabilisation without direct interaction - Université Claude Bernard Lyon 1 Accéder directement au contenu
Article Dans Une Revue International Journal of Molecular Sciences Année : 2020

Human serum albumin in the presence of AGuIX nanoagents: Structure stabilisation without direct interaction

Résumé

The gadolinium-based nanoagent named AGuIX ® is a unique radiosensitizer and contrast agent which improves the performance of radiotherapy and medical imaging. Currently tested in clinical trials, AGuIX ® is administrated to patients via intravenous injection. The presence of nanoparticles in the blood stream may induce harmful effects due to undesired interactions with blood components. Thus, there is an emerging need to understand the impact of these nanoagents when meeting blood proteins. In this work, the influence of nanoagents on the structure and stability of the most abundant blood protein, human serum albumin, is presented. Synchrotron radiation circular dichroism showed that AGuIX ® does not bind to the protein, even at the high ratio of 45 nanoparticles per protein at 3 mg/L. However, it increases the stability of the albumin. Isothermal thermodynamic calorimetry and fluorescence emission spectroscopy demonstrated that the effect is due to preferential hydration processes. Thus, this study confirms that intravenous injection of AGuIX ® presents limited risks of perturbing the blood stream. In a wider view, the methodology developed in this work may be applied to rapidly evaluate the impact and risk of other nano-products that could come into contact with the bloodstream.
Fichier principal
Vignette du fichier
ijms-21-04673-v2.pdf (2.53 Mo) Télécharger le fichier
Origine : Publication financée par une institution
Loading...

Dates et versions

cea-02886720 , version 1 (01-07-2020)

Identifiants

Citer

Xiaomin Yang, Marta Bolsa-Ferruz, Laurent Marichal, Erika Porcel, Daniela Salado-Leza, et al.. Human serum albumin in the presence of AGuIX nanoagents: Structure stabilisation without direct interaction. International Journal of Molecular Sciences, 2020, 21 (13), pp.4673. ⟨10.3390/ijms21134673⟩. ⟨cea-02886720⟩
96 Consultations
81 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More